The Battle of Puná, a peripheral engagement of Francisco Pizarro's conquest of Peru, is fought in April 1531 on the island of Puná (in the Gulf of Guayaquil) in Ecuador.

Pizarro's conquistadors, boasting superior weaponry and tactical skill, decisively defeat the island's indigenous inhabitants.

The battle marks the beginning of Pizarro's third and final expedition before the fall of the Inca Empire.