The Portuguese invasion of Jaffna kingdom in 1560 is the first expedition against the Jaffna kingdom by the Portuguese Empire.

It is led by Viceroy Dom Constantino de Bragança and results in the capture of the capital, Nallur.

The king of Jaffna, Cankili I, manages to escape and regains the capital through a pact that he makes with the Portuguese.

He subsequently incites a peoples' rebellion against the Portuguese, resulting in their withdrawing their forces from Nallur.

The Jaffna kingdom, however, loses its sovereignty over Mannar Island and its main town, Mannar.