The Real Audiencia of Quito (sometimes referred to as la Presidencia de Quito or el Reino de Quito) is an administrative unit in the Spanish Empire that has political, military, and religious jurisdiction over territories that today include Ecuador, parts of northern Peru, parts of southern Colombia and parts of northern Brazil.

It is created by Royal Decree on August 29, 1563 by Philip II of Spain in the city of Guadalajara (Law X of Title XV of Book II of the Recopilación de Leyes de Indias).

It ends in 1822 with the incorporation of the area into the Republic of Gran Colombia, which had been established in 1819.