São Tomé is additionally harboring slave trade relationships intermittently with Angola and the Kingdom of Kongo throughout the early to mid sixteenth century.

Furthermore, in 1525 São Tomé had begun its slave trade relationship with the Spanish Americas.

Most of the slaves to the Spanish Americas go to the Caribbean and Brazil.

In the period between 1532 and 1536, every year São Tomé sends an average of three hundred and forty-two slaves to the Antilles.

Prior to 1580, the island will account for seventy-five percent of Brazil's imports, mainly exporting slaves.

The slave trade will remain a cornerstone of São Tomé's economy until the beginning of the seventeenth century.