Lattanzio Gambara, a native of Brescia, had as a fifteen-year-old initially apprenticed with Giulio Campi in Cremona; by 1549, he was working alongside Girolamo Romanino, who had eventually become his father-in-law.

An altarpiece of S. Maria in Silva dates to 1558.

He frescoes for the Villa Contarini in Asolo, and shows the influenced of Il Pordenone.

He paints a cycle of frescoes on the History of the Apocalypse that will decorate the Loggia of Brescia until they are destroyed by bombing in 1944.

The artist has returned to Brescia in these years to work with Romanino in a series of generally lost frescoes for Sant'Eufemia and Saint Lorenzo in Brescia.

He has painted altarpieces for the abbey of Saint Benedict in Polirone.

He has also decorated Palazzo Mayo in Cadignano (Lama Mocogno, in collaboration with Giulio and Antonio Campi).